Foxn1^em2Oers

Fifteen nucleotides (GAAGAGGAAAGACCC) were deleted from exon 7 using an sgRNA and an ssODN template with CRISPR/Cas9 technology, resulting in a disruptive in-frame deletion and codon change (p.W363_P368delinsC). The mutation replicates a human mutation (GAAGAGGAAAGATCC deletion) associated with thymic-hypoplasia-linked SCID without alopecia or nail dystrophy when compound heterozygous with another mutation in the same gene (represented by the Foxn1em1Oers allele). (J:280961)