Gt(ROSA)26Sor^{tm2(CAG-mCherry/CHRM4*)Rray}

This mouse line is responsive to Cre and FLP recombinases. Upon FLP recombination, cells will express mCherry. Upon Cre/FLP dual recombination, cells will express hM4D. It can be used to delineate cell types by dual expression of Cre and FLP recombinases through transgenic transmission or virus. Upon dual recombination, intersectional targeted cells will express hM4D, a modified hM3 receptor that can be activated using Clozapine-N-Oxide (CNO), a synthetic drug, for Gi-mediated perturbation of cells. It is useful in studies on the role of any cells defined by Cre/FLP in a variety of behavioral and physiological assays with high spatial and temporal resolution. (J:94077)