Ido2^em1Lamn

Arginine codon 235 (CGG) in exon 8 was changed to tryptophan (TGG) (p.R235W) using sgRNAs (targeting CTTACCCAGAGAGGAAGATC, GTCATCCGGATCTTCCTCTC, and TCATCCGGATCTTCCTCTCT) and an ssODN template with CRISPR/Cas9 technology. The mutation is the equivalent of common human SNP rs10109853 (p.R248W) that renders the encoded peptide catalytically inactive. (J:342726)