Tnrc18^em1Ggw

Tryptophan codon 2563 (TGG) and tyrosine codon 2565 (TAC) in exon 23 were changed to alanine (GCC, GCC) (ENSMUSP00000117651:p.W2563A, p.Y2565A) using an sgRNA (targeting GTGGTTCTACCATCCGG) and an ssODN template with CRISPR/Cas9 technology. The mutations are the equivalent of the human p.W2858A and p.Y2860A mutations and render the encoded peptide H3K9me3-binding-defective. (J:342966)