Snca^{tm3.1(SNCA*E46K)Mdk}

The targeting vector is designed to insert a 158 kb (Chr4:89707185 - 89865027) fragment of the human SNCA carrying the E46K (glutamic acid to lysine) mutation in exon 3 in place of Chr6:60683985 - 60848702 of the mouse Snca gene. The genomic sequences are from human assembly GRCh38.p14 and the mouse assembly GRCm39. The construct includes an loxP-flanked neomycin selection cassette. In addition, there is also a SNCA non-coding antisense RNA in the human genome segment. The insertion disrupts Snca transcription. (J:101977)