Snca^{tm2.1(SNCA*A53T)Mdk}

The targeting vector is designed to insert a 158 kb (Chr4:89707185 - 89865027) fragment of the human SNCA carrying the A53T (alanine to threonine) mutation in place of Chr6:60683985-60848702 of the mouse Snca gene. The genomic sequences are from human assembly GRCh38.p14 and the mouse assembly GRCm39. The construct includes an loxP-flanked neomycin selection cassette. In addition, there is also a SNCA (non-coding antisense RNA) in the human genome segment. The insertion disrupts Snca transcription. Transient cre-medited recombination removed the neo cassette. (J:101977)