Ccdc28b^em1Jlba

CRISPR/Cas9 technology targeting the 5 end of exon 3 generated two one base pair deletions, and is predicted to result in a frameshift and premature stop codon. Exon 3 is shared by all transcripts and coding isoforms. Real-time qPCR showed a reduction in mRNA levels, likely due to NMD-mediated degradation of premature stop codon containing mRNA. Western blot analysis shows absence of protein. (J:325766)