在exon 3上游插入了loxP位点,并在neomycin抗性基因周围嵌入了自剪切锚定位点lox2272,接着是反向排列的SA-2A-dTomato-polyA元件。exon 3下游的内含子3中,又插入了另一个loxP和lox2272位点。这个neomycin抗性基因经历了自剪切。通过Cre介导的重组,使用loxP位点,可能将dTomato元件和exon 3颠倒过来,使得dTomato位于exon 3上游,或者仅通过lox2272将dTomato元件反转。使用loxP的Cre介导剪切会删除exon 3。来源:(J:329964)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Not Specified
Targeted
插入,基因内删除
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1
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1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

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PMID
Journal
Year
IF
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