在exon 3的上游插入了一个loxP位点,并在neomycin抗性基因周围设计了自剪切锚定位点-lox2272,接着是反向排列的SA-2A-dTomato-polyA插件,随后在exon 3的外显子3下游,再插入了另一个loxP和lox2272位点。这个neomycin抗性基因通过自剪切被去除了。利用lox2272的Cre介导重组,反转了dTomato序列。(来源:J:329964)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Not Specified
Targeted
插入
--
1
--
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

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PMID
Journal
Year
IF
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