Tnfrsf1a^em1Tomm

Threonine exon 79 (ACA) in exon 3 was changed to methionine (ATG) (p.T79M) using a crRNA (targeting CAGGGCGGGATACAGTCTGCAGG) and an ssODN template with CRISPR/Cas9 technology. The mutation is the equivalent of the same human mutation associated with tumor necrosis factor (TNF) receptor-associated periodic syndrome (TRAPS). (J:342733)