Snca^{tm2.1(SNCA*)Ge}

A targeting vector consisting of loxP-flanked human alpha-synuclein, followed by a C-terminally truncated human alpha-synuclein lacking 21 amino acids, IRES, a placental alkaline phosphatase (pLAP)reporter gene, and an FRT-flanked neomycin gene was targeted to exon 3. The neomycin selection cassette was removed via flp-mediated recombination. Cre-mediated recombination removed the human alpha-synuclein resulting in the expression of the C-terminally truncated protein. RT-PCR confirmed the expression of the expected 503 bp human mutant transcript. The truncation terminates with aspartic acid at residue 119 (120 CCT to TAA) and favors fibrillary aggregate formation. (J:314280)