Otof^em1Bros

This mouse line was generated by site-directed CRISPR-Cas9 mutagenesis in C57BL/6NCrl mice causing a I1553T mutation (uniprot Q9ESF1-3). This mimics the mutation that gives rise to a deafness related I1573T mutation in the isoform e peptide coded for by the human ortholog. Additional silent mutations were introduced. The mutated exon38 now reads:(5')gtcctttgacattgaggcctccttccccatggagtccatgttgacagtggccgtgtacgactgggatctggtgggcactgatgaTctcaCcggCgaaaccaagattgacctggaaaaccgcttctacagcaagcaCcgcgcAacAtgcggcatcgcacagacctattccat(3'). (J:343835)