Tg(tetO-lacZ,-SHANK2_iA*,-Venus)2Rsp

The transgene contains a bi-directional tetracycline-responsive promoter element controlling the expression of the nuclear-localized beta-galactosidase (nLacZ) on one side and fusion transcript composed of green fluorescent protein variant (Venus), fused via the ribosomal skipping sequence T2A to the brain-specific isoform (A) of the human gene with the mutation responsible for the amino acid substitution of arginine with a stop codon at position 462 (R462*). (J:310967)