Pcdh15^em1Zma

Arginine codon 250 (CGA) in exon 9 was changed to a stop codon (TGA) (p.R250*) using an sgRNA and an ssODN template with CRISPR/Cas9 technology. The mutation is the equivalent of the human p.R245* mutation associated with Usher syndrome type I (USH1). Immunohistochemistry experiments indicate absence of protein expression in retinal and cochlear tissues. (J:342718)