Gt(ROSA)26Sor^{tm1(CAG-mEGFP,-YFP*)Cheny}

The targeting construct contains a CAG-reversed [loxP-pA-PGK-Neomycin-STOP-loxP]-FLIM-AKAR-WPRE cassette was introduced to intron 1. The Fluorescence Lifetime Imaging Microscopy compatible A Kinase Activity Reporter (FLIM-AKAR) optical reporter (based on the synthetic reporter found in Addgene plasmid# 63058) consists of a donor fluorophore of monomeric enhanced green fluorescent protein (mEGFP) and an acceptor fluorophore of dark yellow fluorescent protein (sREACH). (J:343075)