Idh1^tm1.1Avd

A loxP site was inserted into intron 2, and a lox511 site, and, in reverse orientation, a copy of exon 3 (where arginine codon 132 (CGA) was changed to histidine (p.R132H)), a second loxP site and a second lox511 site, were inserted into intron 3. An FRT site flanked selection cassette that was inserted between the second loxP and lox511 sites was removed through subsequent Flp-mediated recombination. This allele expresses the wildtype peptide and only after conditional Cre-mediated flipping of the mutated exon copy and removal of the wildtype exon will it express the modified peptide. The mutation represents the most common variant found in human gliomas. (J:342699)