Zp2^em1Zbw

CRISPR/Cas9 technology generated a CT to TG substitution (c.1622-1623CT>TG) resulting in a threonine to methionine change at amino acid 541 (p.T541M) in exon 14. A c.1632G>A synonymous mutation was also introduced. This corresponds to the p.Thr539Met mutation identified in a human female with primary infertility. (J:342437)