Sema4d^em1Xiji

Lysine codon 848 (AAG) in exon 16 (in ENSMUST00000021900) was changed to threonine (ACG) (p.K848T) using an sgRNA and an ssODN template with CRISPR/Cas9 technology. The mutation is the equivalent of the human p.K849T mutation which has been identified as the first causal mutation for primary sclerosing cholangitis (PSC). (J:302974)