CRISPR/Cas9技术产生了一个19个碱基对的缺失(CTTCACAGACCTGACTCGCC),导致了框移和早停密码子,这在同源纯合子的小肠匀浆蛋白检测中通过 Western blot得到了确认。(来源:J:342295)
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CRISPR/Cas9 technology generated a 19-bp deletion (TTCACAGACCTGACTCGCC) in exon 2, resulting in a frame-shift and premature stop codon. Western blot analysis confirmed absence of protein in ileum lysates of homozygotes. (J:342295)