CRISPR/Cas9 technology using gRNAs 5-AAAAGATGAAATTGAAGAGA-3 for the second exon and 5-ATTTATGACAGCGATAGCTC-3 for the sixth exon, generated a 12,565-bp deletion of exons 2-6. Western blot analysis confirmed absence of protein in testicular germ cells of homozygotes. (J:338084)