An FRT-flanked PGK-neo cassette and a loxP site were inserted upstream of exon 2 and a second loxP site was inserted downstream of exon 5. The neomycin cassette was removed via flp-mediated recombination, leaving exons 2-5 floxed. (J:342039)
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An FRT-flanked PGK-neo cassette and a loxP site were inserted upstream of exon 2 and a second loxP site was inserted downstream of exon 5. The neomycin cassette was removed via flp-mediated recombination, leaving exons 2-5 floxed. (J:342039)