在一段由loxP引导的PGK-抗氨苄西林抗性基因取代了第1和2号外显子之间的3.3kb内含子,打断了开放阅读框。 Western blot结果显示大脑中没有检测到蛋白质(来源:J:341520)
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A loxP-flanked PGK-neomycin resistance gene replaced the 3.3-kb intron between exons 1 and 2, disrupting the open reading frame. Western blot analysis confirmed the absence of protein in the brain. (J:341520)