在exon 3的上游插入了一个loxP位点,通过CRISPR/Cas9技术,又插入了一个FRT标记的PGK-neomycin cassette和第二个loxP位点在exon 3的下游。随后通过Flp介导的重组,去除了选择性 cassette,留下exon 3是可剪接的。(来源:J:314515)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
C57BL/6J
Endonuclease-mediated
插入
--
1
2
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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