这个等位基因是通过首先插入一个Actb-neo cassette,然后在116,237,220(在mm9的Chromosome 12上)位置添加一个loxP(fl)位点,接着插入Bsd-rox的抗布氏放线菌素抗性基因,接着在VH基因簇的中间插入frt-Pgke启动子序列(frt)和另一个lox(lox71)位点(同向),最后在117,126,667位置插入Puro cassette在Igh的5'区域实现的。(来源:J:341427)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
(C57BL/6 x 129P2/OlaHsd)F1
Targeted
插入
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--
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1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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