Ank2^tm1.2Csbd

The targeting vector is designed to contain (from 5'; to 3') an FRT site, an En2 splice acceptor, an internal ribosome entry site (IRES) fused to an EGFP sequence with a polyadenylation signal, and a floxed cassette containing a neo and a second FRT site all upstream of exon 4. A third loxP was placed downstream of exon 4. FLP and Cre-mediated recombination in the germline resulted in the deletion of exon 4. (J:340358)