RMCE通过重组插入了cre-依赖的FLEX(翻转剔除)元件,其中包含了一个CAG启动子在FLEX元件之前,FLEX元件内是hM4Di-mKateT的编码序列,但方向与启动子相反。FLEX元件的下游是反向插入的兔球蛋白polyA序列,每个读框都有终止密码子。hM4D的ORF后面跟着6xHis标签和V5标签,但这些在固定组织的免疫组化中并未检测到。(来源:J:304133)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
(C57BL/6J x 129S6/SvEvTac)F1
Targeted
插入
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1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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