Igs7^{em1(CAG-mCherry)Fcam}

The targeting construct contains the mCherry reporter open reading frame with a WPRE and the 10 target sequences (see below) inserted into the 3 UTR driven by a CAG promoter and inserted into the Igs7 locus using CRISPR/cas9 genome editing. A "barcode" 276 bp array containing 10 target sites in a unique arrangement is inserted upstream of the EGFP polyadenylation site. (J:341616)