Gt(ROSA)26Sor^{em1.1(CAG-EGFP*)Maoh}

The targeting construct inserted an FRT site, CAG promoter driving ubiquitous expression of an EGFP reporter gene, FRT site, and lox2272 site. Further CRISPR/Cas9 genome editing introduced a 1bp deletion in the EGFP gene, eliminating expression of the reporter. (J:340084)