这个靶向构建设计用于在Pld2基因的Exon11内上游插入一个loxP位点,随后是FRT框引导的neo cassette,再在Exon12的下游插入另一个loxP位点。通过Flp介导的重组,会移除neo cassette,使得Exons11和12区域被标记为可靶向敲除。(来源:J:260679)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count