Gt(ROSA)26Sor^{tm1(Sirt6)Mpgu}

The targeting vector was designed to insert the Sirt6-FLAG cDNA into the Gt(ROSA)26Sor driven by its promoter and controlled by a STOP cassette. The STOP cassette in the vector consisted of a splice acceptor and the floxed (PGK)/gb2neoPGKpolyA2xSV40pA sequence and was inserted in intron 1 of ROSA26 locus. Mouse Sirt6 cDNA with a C-terminal FLAG tag was cloned immediately downstream of the STOP cassette. The Sirt6-FLAG sequence was followed by a BGHpA bovine growth hormone polyadenylation (BGHpA) sequence. (J:338782)