The targeting construct contains the phage P1 loxP sites, SA, mouse F8 cDNA, Simian Virus (SV40) PolyA, Saccharomyces cerevisiae Frt site, P2A (porcine teschovirus-1 2A peptide), jellyfish EGFP cDNA, and mouse F8 genomic DNA. Flp-mediated recombination removed the selection cassette. cre-mediated recombination removed the floxed segment. (J:104881)