The transgene contains the mouse genomic DNA[Mouse BAC clone (R23-261I9)], human Myc, Synthetic His-tag, Bovine BGH polyA, human ACTB polyA, and yeast FRT (flippase recombination target) site. The amino acid sequence after Tyr at 163 aa was replaced with Myc-His tag in BAC clone (R23-261I9). (J:104881)