CRISPR/Cas9技术用GFP序列替换了大部分exon 2和整个exon 3。虽然GFP转录物丰度高,但通过 Western blotting 或免疫染色在视网膜中未检测到GFP蛋白。(来源:J:339742)
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CRISPR/Cas9 technology replaced most of exon 2 and the entire exon 3 with a GFP sequence. While abundant expression of GFP transcripts is seen, no GFP protein is detected in retinas by Western blotting or immunostaining. (J:339742)