CRISPR/cas9 genome editing used guide RNAs [CCC CCGGGATGGCTCTGATATA] to insert an GSG-P2A peptide sequence and mVenus fluorescent protein coding sequence into the 3' UTR. Foxi3 transcript Foxi3-201 (ENSMUST00000069634.6) was used as reference for the exon number and guide sequences. Donor DNA contained two nucleotide changes (C to T and C to G) to prevent the inserted sequence from being targeted by the Foxi3 gRNA. (J:339365)