在基因c.1145-1148(GRCm39:chr10:g.75732893-75732896)的一个cDNA序列中,通过CRISPR/Cas9技术,目标是用一个crRNA(针对GCCAACACTGCCCCAGCC)和一个ssODN模板(GGCGAATGAGGCGTCTTGCCAACACTGCCCAGCCTGGTGATGAAGACATCCATGCTCGAC)进行删除。这个删除导致了在终止密码子前的框移,使得最后3个内源性密码子被35个非内源性密码子替换。(来源:J:337790)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(C57BL/6J x DBA)F1
Endonuclease-mediated
基因内删除
--
1
13
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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