The transgene contains the mouse promoter driving expression of GCaMP6 (RSET tag, chicken smooth muscle M13, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, Rat calmodulin (CaM) cDNA(aa.2-148)) fused with human ACTB gene, Foot-and-mouth disease virus (FMDV derived) 2A peptide (F2A), Discosoma sp. mCherry cDNA, Woodchuck Hepatitis Virus Post transcriptional Regulatory Element (WPRE), and mouse polyadenylation sequence. (J:297280)