This allele was generated at The Centre for Phenogenomics by injecting Cas9 mRNA with a guide RNA with the spacer sequence AGCATCTGACACTGAGTAAGand a single-strand oligonucleotide encoding the changes c.1376_1377CA>TC (p.S459F) and a silent mutation c.1368C>T (p.A456) to disrupt the protospacer adjacent motif (PAM) sequence. (J:339146)