Pla2g4a^tm1.1Cect

The targeting construct inserted into intron three a loxP site-flanked puromycin resistance gene followed by a premature stop codon and a modified exon 4 with the mutation responsible for the amino acid substitution of arginine, lysine, and arginine to alanine at positions 57, 58, and 59 (R57A, K58A, and R59A) in the C1P binding site. Cre-mediated recombination removed the selection cassette and stop. (J:295310)