RMCE通过重组酶介导的 cassette exchange 把 Gt(ROSA)26Sortm1Maoh 基因座中JT15和lox2272之间的表达 cassette 替换成了CAG启动子-rox-EGFP-poly(A)-CAT(氯霉素乙酰转移酶)-poly(A)-rox-tdTomato-poly(A) cassette。Flp介导的重组移除了FRT端点周围的额外序列,而Dre介导的重组则去除了rox标记的EGFP-poly(A)-STOP序列。(来源:J:303408)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count