RMCE通过重组酶介导的 cassette exchange 把 Gt(ROSA)26Sortm1Maoh 基因座中JT15和lox2272之间的表达 cassette 替换成了CAG启动子-rox-EGFP-poly(A)-CAT(氯霉素乙酰转移酶)-poly(A)-rox-tdTomato-poly(A) cassette。Flp介导的重组移除了FRT端点周围的额外序列,而Dre介导的重组则去除了rox标记的EGFP-poly(A)-STOP序列。(来源:J:303408)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
129P2/OlaHsd
Targeted
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1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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