Foxo1^tm1Zzhu

A loxP site, a Foxo1 cDNA, a roxP site, a second loxP site, and poly(A) signal sequence, were inserted into the 5' UTR of a mutated exon 1. The mutation involved changing threonine codon 24 (ACC) to alanine (p.T24A). This allele expresses the wild-type transcript, but after Cre-mediated deletion of the cDNA sequence it expresses the mutated transcript. The mutation changes a phosphorylatable residue in the encoded peptide to a phosphoblocker, which blocks the inactivation of the protein. (J:287219)