A loxP site and FRT flanked pgk-neo cassette were inserted upstream of exon 2 and a loxP site was inserted downstream of exon 2 via homologous recombination. Flp mediated recombination removed the neo cassette. (J:338296)
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A loxP site and FRT flanked pgk-neo cassette were inserted upstream of exon 2 and a loxP site was inserted downstream of exon 2 via homologous recombination. Flp mediated recombination removed the neo cassette. (J:338296)