Fgg^tm1.1Geno

Glutamine codons 423 and 424 (CAG) in exon 9 were changed to asparagine (p.Q423N, p.Q424N), lysine codon 431 (AAA) to arginine (p.K431R) and a loxP site flanked neomycin resistance gene cassette was inserted into intron 8. The neo cassette was removed through subsequent Cre-mediated recombination. The mutations eliminate the gamma-chain cross-linking sites and recapitulate the same experimentally created mutations in the human ortholog (p.Q398N p.Q399N, p.K406R in the mature peptide) that affect blood clot formation. (J:307658)