这个靶向载体被设计成在exon 3上游插入一个含loxP切割位点的PMC1-诺卡因插件,然后是exon 12下游的loxP切割位点、PGK-溴脱氧尿嘧啶核苷酸(puromycin)插件和一个splice接受器序列,最后是lacZ报告基因。来自Ctcf条件性小鼠的ES细胞被新分离出来,并用表达Cre重组酶的 lentivirus 感染。筛选出的ES细胞不含exon 3上游的诺卡因插件,但保留了3-12号exons的loxP标记,以及下游的loxP标记的PGK-puromycin插件和lacZ报告基因。(来源:J:143271)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129P2/OlaHsd
Targeted
插入
--
1
2
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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