Dnmt1^em2Jiemw

Proline codon 497 (CCC) was changed to tyrosine (TAC) (p.P497Y) using an sgRNA and an ssODN template with CRISPR/Cas9 technology. The mutation mimics the human p.P491Y mutation associated with HSAN1E (hereditary sensory and autonomic neuropathy type 1E with dementia and hearing loss). (J:310870)