Dnmt1^em1Jiemw

Tyrosine codon 501 (TAT) was changed to cysteine (TGT) (p.Y501C) using an sgRNA and an ssODN template with CRISPR/Cas9 technology. The mutation mimics the human p.Y495C mutation associated with HSAN1E (hereditary sensory and autonomic neuropathy type 1E with dementia and hearing loss). (J:310870)