Sftpc^tm1.1Twv

The following elements were inserted immediately downstream of exon 1: coding cDNA sequence for exons 2-5 where exon 5 has a T-to-A point mutation changing leucine codon 184 to glutamine (p.L184Q), triple stop codons, bovine growth hormone gene poly(A) signal sequences and a loxP site flanked neomycin resistance gene cassette. The neo cassette was removed through subsequent Cre-mediated recombination. The mutation mimics the p.L188Q human mutation associated with lung disease. (J:313007)