A nuclear tdTomato reporter was inserted immediately upstream of the coding region in exon 3 of the gene. The reporter is followed by an A2 self-cleavable peptide, allowing independent production of the tdTomato and the IL-18BP proteins. An frt-flanked neomycin selection cassette was inserted into intron 7, then subsequently removed after selection via FLP-mediated recombination in the germline. (J:337716)