Tg(CAG-cre)fKymm

The allele was generated in the TT2-derived ES cell clone 71-5F2-23 by targeted replacement via RMCE in unknown Igs54 (integrated site) in the mouse locus. The original ES cell clone integrated the CAG-lox71-bsr-pA-lox511-FRT-lox2272 target lox sites. The bsr gene was replaced with the lox66-cre-pA-MC1neopA-lox2272 expression cassette through Cre-mediated recombination so that the ubiquitous CAG promoter drives Cre recombinase expression. After RMCE, ES cell clone line f was selected and generated chimera, and germline transmission was confirmed. (J:243719)