The transgene contains the a crown cell-specific enhancer derived from the mouse Nodal gene driving expression of cilia-targeted GCaMP6 and mKeima reporter genes (5-hydroxytryptamine receptor isoform 6; 5HT6/GCaMP6, 5HT6/mKeima). GCaMP6 contains a RSET tag, chicken smooth muscle M13, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, and rat calmodulin (CaM) cDNA (aa.2-148). Line 3A was generated. (J:336740)