The bacterial artificial chromosome, RP23-113I19, containing the Gm5127 predicted gene (including 27 kb of 5' upstream and 70 kb of 3' downstream) was modified by recombineering to insert an inducible cre recombinase/ERT2 cassette (pCAG-CreERT2) and a FRT flanked neomycin cassette (with 50 bp overhangs) into the start codon of the Gm5127 gene sequence. (J:336605)